miR-203 Inhibits the Migration and Invasion of Esophageal Squamous Cell Carcinoma by Regulating LASP1

Int J Oncol. 2012 Nov;41(5):1653-61. doi: 10.3892/ijo.2012.1614. Epub 2012 Aug 30.

Abstract

The expression of microRNA-203 (miR-203) in esophageal squamous cell carcinoma (ESCC) tissues is remarkably lower than that in non‑ESCC tissues. We investigated how miR-203 could influence the development of ESCC cells. Our analyses revealed that miR-203 inhibited the migration and invasion of ESCC cells. Genome-wide gene expression data and target site inhibition assays showed that miR-203 appears to directly regulate LIM and SH3 protein 1 (LASP1). The knockdown of LASP1 resulted in inhibition of the migration and invasion of ESCC cells. Our results suggest that miR-203 and its target LASP1, may be associated with the progression of ESCC. In clinical ESCC specimens, the expression levels of miR-203, which were lower compared to those in normal tissues, were inversely correlated with the mRNA expression levels of LASP1. Moreover, we found that there was a significant correlation between the expression levels of miR-203 and the relapse‑free survival. The identification of a cancer network regulated by miR-203 could provide new insights into the potential mechanisms of the progression of ESCC.

MeSH terms

  • 3' Untranslated Regions
  • Adaptor Proteins, Signal Transducing / genetics*
  • Base Sequence
  • Binding Sites
  • Carcinoma, Squamous Cell / genetics*
  • Cell Line, Tumor
  • Cell Movement / genetics*
  • Cell Proliferation
  • Cytoskeletal Proteins / genetics*
  • Esophageal Neoplasms / genetics*
  • Gene Expression
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • LIM Domain Proteins / genetics*
  • MicroRNAs / genetics*
  • Transfection

Substances

  • 3' Untranslated Regions
  • Adaptor Proteins, Signal Transducing
  • Cytoskeletal Proteins
  • LASP1 protein, human
  • LIM Domain Proteins
  • MIRN203 microRNA, human
  • MicroRNAs