A specific oligodeoxynucleotide promotes the differentiation of osteoblasts via ERK and p38 MAPK pathways

Int J Mol Sci. 2012;13(7):7902-14. doi: 10.3390/ijms13077902. Epub 2012 Jun 25.

Abstract

A specific oligodeoxynucleotide (ODN), ODN MT01, was found to have positive effects on the proliferation and activation of the osteoblast-like cell line MG 63. In this study, the detailed signaling pathways in which ODN MT01 promoted the differentiation of osteoblasts were systematically examined. ODN MT01 enhanced the expression of osteogenic marker genes, such as osteocalcin and type I collagen. Furthermore, ODN MT01 activated Runx2 phosphorylation via ERK1/2 mitogen-activated protein kinase (MAPK) and p38 MAPK. Consistently, ODN MT01 induced up-regulation of osteocalcin, alkaline phosphatase (ALP) and type I collagen, which was inhibited by pre-treatment with the ERK1/2 inhibitor U0126 and the p38 inhibitor SB203580. These results suggest that the ERK1/2 and p38 MAPK pathways, as well as Runx2 activation, are involved in ODN MT01-induced up-regulation of osteocalcin, type I collagen and the activity of ALP in MG 63 cells.

Keywords: ERK1/2 MAPK; differentiation; oligodeoxynucleotide; osteoblast; p38 MAPK.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / genetics
  • Alkaline Phosphatase / metabolism
  • Butadienes / pharmacology
  • Cell Differentiation / drug effects*
  • Cell Line
  • Collagen Type I / genetics
  • Collagen Type I / metabolism
  • Core Binding Factor Alpha 1 Subunit / genetics
  • Core Binding Factor Alpha 1 Subunit / metabolism
  • Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Humans
  • Imidazoles / pharmacology
  • MAP Kinase Signaling System*
  • Nitriles / pharmacology
  • Oligodeoxyribonucleotides / metabolism
  • Oligodeoxyribonucleotides / pharmacology*
  • Osteoblasts / drug effects
  • Osteoblasts / physiology*
  • Osteocalcin / genetics
  • Osteocalcin / metabolism
  • Pyridines / pharmacology
  • Transcriptional Activation / drug effects
  • p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Butadienes
  • Collagen Type I
  • Core Binding Factor Alpha 1 Subunit
  • Imidazoles
  • Nitriles
  • ODN MT01
  • Oligodeoxyribonucleotides
  • Pyridines
  • RUNX2 protein, human
  • U 0126
  • Osteocalcin
  • Extracellular Signal-Regulated MAP Kinases
  • p38 Mitogen-Activated Protein Kinases
  • Alkaline Phosphatase
  • SB 203580