Label-free detection of DNA hybridization using transistors based on CVD grown graphene

Biosens Bioelectron. 2013 Mar 15;41:103-9. doi: 10.1016/j.bios.2012.07.059. Epub 2012 Aug 17.

Abstract

The high transconductance and low noise of graphene-based field-effect transistors based on large-area monolayer graphene produced by chemical vapor deposition are used for label-free electrical detection of DNA hybridization. The gate materials, buffer concentration and surface condition of graphene have been optimized to achieve the DNA detection sensitivity as low as 1 pM (10(-12) M), which is more sensitive than the existing report based on few-layer graphene. The graphene films obtained using conventional PMMA-assisted transfer technique exhibits PMMA residues, which degrade the sensing performance of graphene. We have demonstrated that the sensing performance of the graphene samples prepared by gold-transfer is largely enhanced (by 125%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Conductometry / instrumentation*
  • Crystallization / methods*
  • DNA / analysis
  • DNA / genetics*
  • Electrodes
  • Equipment Design
  • Equipment Failure Analysis
  • Gases / chemistry
  • Graphite / chemistry*
  • Nucleic Acid Hybridization / genetics*
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Staining and Labeling
  • Transistors, Electronic*

Substances

  • Gases
  • Graphite
  • DNA