Estradiol and estrogen receptor-dependent stabilization of a minivitellogenin mRNA lacking 5,100 nucleotides of coding sequence

Mol Cell Biol. 1990 Jan;10(1):371-6. doi: 10.1128/mcb.10.1.371-376.1990.

Abstract

We have developed a transfection assay to investigate the estrogen-mediated stabilization of cytoplasmic vitellogenin mRNA. A minivitellogenin (MV5) gene containing the 5' and 3' untranslated and coding regions but lacking 5,075 nucleotides of internal coding sequence was constructed. Cotransfection of the MV5 plasmid and a Xenopus estrogen receptor expression plasmid into Xenopus liver tissue culture cells yielded a 529-nucleotide MV5 mRNA, which was specifically stabilized by estrogen. MV5 mRNA exhibited the increased stability indicative of positive regulation when the estradiol-estrogen receptor complex was present and was not destabilized by unliganded estrogen receptor. Transfected estrogen receptor, estradiol, and 529 nucleotides of the 5,604-nucleotide vitellogenin B1 mRNA were sufficient for stabilization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cloning, Molecular
  • Estradiol / pharmacology*
  • Gene Expression Regulation / drug effects
  • Oocytes / physiology*
  • RNA, Messenger / genetics*
  • Receptors, Estrogen / physiology*
  • Restriction Mapping
  • Structure-Activity Relationship
  • Transfection
  • Vitellogenins / genetics*
  • Xenopus laevis

Substances

  • RNA, Messenger
  • Receptors, Estrogen
  • Vitellogenins
  • Estradiol