Omega-3 fatty acid-derived mediator, Resolvin E1, ameliorates 2,4-dinitrofluorobenzene-induced atopic dermatitis in NC/Nga mice

Int Immunopharmacol. 2012 Dec;14(4):384-91. doi: 10.1016/j.intimp.2012.08.005. Epub 2012 Aug 27.


Atopic dermatitis (AD) is a common inflammatory skin disease for which few effective treatments are available. Resolvin E1 (RvE1; 5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid) is an endogenous lipid mediator derived from omega-3 fatty eicosapentaenoic acid, which is a potent inhibitor of inflammation. AD-like skin lesion was induced by repetitive skin contact with DNFB in NC/Nga mice and the effects of RvE1 were evaluated on the basis of histopathological findings of skin, ear swelling and cytokine production of CD4(+) T cells. Intraperitoneal injection of RvE1 for one week after DNFB challenge significantly lowered ear swelling and improved back skin lesions. In addition, RvE1 significantly suppressed production of interferon-gamma (IFN-γ) and interleukin-4 (IL-4) by activated CD4(+) T cells and serum IgE level. Furthermore, RvE1 reduced DNFB-induced infiltration of eosinophils, mast cells, CD4(+) T cells, and CD8(+) T cells in skin lesions. Therefore, RvE1 may suppress the development of AD-like skin lesions in DNFB-treated NC/Nga mice by reducing IL-4 and IFN-γ of activated CD4(+) T cells and serum IgE levels and infiltration of immune cells to skin lesion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / metabolism
  • Dermatitis, Atopic / chemically induced*
  • Dermatitis, Atopic / drug therapy*
  • Dinitrofluorobenzene / toxicity*
  • Dose-Response Relationship, Drug
  • Eicosapentaenoic Acid / analogs & derivatives*
  • Eicosapentaenoic Acid / chemistry
  • Eicosapentaenoic Acid / pharmacology
  • Fatty Acids, Omega-3 / chemistry*
  • Immunoglobulin E / blood
  • Interferon-gamma / genetics
  • Interferon-gamma / metabolism
  • Interleukin-4 / genetics
  • Interleukin-4 / metabolism
  • Male
  • Mice
  • Mice, Inbred Strains
  • Skin / pathology


  • Fatty Acids, Omega-3
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma
  • Eicosapentaenoic Acid
  • Dinitrofluorobenzene
  • 5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid