Evidence that the Arabidopsis Ubiquitin C-terminal Hydrolases 1 and 2 associate with the 26S proteasome and the TREX-2 complex

Gang Tian; Qing Lu; Susanne E Kohalmi; Steven J Rothstein; Yuhai Cui

doi:10.4161/psb.21899

Evidence that the Arabidopsis Ubiquitin C-terminal Hydrolases 1 and 2 associate with the 26S proteasome and the TREX-2 complex

Plant Signal Behav. 2012 Nov;7(11):1415-9. doi: 10.4161/psb.21899. Epub 2012 Sep 5.

Authors

Gang Tian¹, Qing Lu, Susanne E Kohalmi, Steven J Rothstein, Yuhai Cui

Affiliation

¹ Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, ON Canada.

Abstract

The 26S proteasome interacts with a number of different proteins, while the TREX-2 complex is an important component of the mRNA export machinery. In animals and yeast, members of the Ubiquitin C-terminal Hydrolase 37 (UCH37) family are found to associate with the 26S proteasome, but this has not been demonstrated in plants. The Arabidopsis UCH1 and UCH2 are orthologous to UCH37. Here, we show that UCH1 and UCH2 interact with the 26S proteasome lid subunits. In addition, the two UCHs also interact with TREX-2 components. Our data suggest that Arabidopsis UCHs may serve as a link between the 26S proteasome lid complex and the TREX-2 complex.

Keywords: 26S proteasome; Arabidopsis; TREX-2 complex; Ubiquitin C-terminal Hydrolase (UCH); deubiquitinating protease.

MeSH terms

Arabidopsis / enzymology*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Proteasome Endopeptidase Complex / genetics
Proteasome Endopeptidase Complex / metabolism*
Protein Binding
Ubiquitin Thiolesterase / genetics
Ubiquitin Thiolesterase / metabolism*

Substances

Arabidopsis Proteins
Ubiquitin Thiolesterase
Proteasome Endopeptidase Complex
ATP dependent 26S protease