Adenosine deaminase polymorphism affects sleep EEG spectral power in a large epidemiological sample

PLoS One. 2012;7(8):e44154. doi: 10.1371/journal.pone.0044154. Epub 2012 Aug 29.

Abstract

Slow wave oscillations in the electroencephalogram (EEG) during sleep may reflect both sleep need and intensity, which are implied in homeostatic regulation. Adenosine is strongly implicated in sleep homeostasis, and a single nucleotide polymorphism in the adenosine deaminase gene (ADA G22A) has been associated with deeper and more efficient sleep. The present study verified the association between the ADA G22A polymorphism and changes in sleep EEG spectral power (from C3-A2, C4-A1, O1-A2, and O2-A1 derivations) in the Epidemiologic Sleep Study (EPISONO) sample from São Paulo, Brazil. Eight-hundred individuals were subjected to full-night polysomnography and ADA G22A genotyping. Spectral analysis of the EEG was carried out in all individuals using fast Fourier transformation of the signals from each EEG electrode. The genotype groups were compared in the whole sample and in a subsample of 120 individuals matched according to ADA genotype for age, gender, body mass index, caffeine intake status, presence of sleep disturbance, and sleep-disturbing medication. When compared with homozygous GG genotype carriers, A allele carriers showed higher delta spectral power in Stage 1 and Stages 3+4 of sleep, and increased theta spectral power in Stages 1, 2 and REM sleep. These changes were seen both in the whole sample and in the matched subset. The higher EEG spectral power indicates that the sleep of individuals carrying the A allele may be more intense. Therefore, this polymorphism may be an important source of variation in sleep homeostasis in humans, through modulation of specific components of the sleep EEG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / genetics*
  • Adult
  • Electroencephalography*
  • Epidemiologic Measurements
  • Female
  • Genotype
  • Humans
  • Male
  • Polymorphism, Single Nucleotide / genetics*
  • Sleep / genetics*
  • Sleep / physiology*
  • Sleep Stages / genetics
  • Sleep Stages / physiology

Substances

  • ADA protein, human
  • Adenosine Deaminase

Grant support

This study was supported by Associação Fundo de Incentivo à Psicofarmacologia (AFIP) and Fundação de Amparo à Pesquisa do Estado de São Paulo (CEPID #98/14303-3). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.