The antimicrobial and osteoinductive properties of silver nanoparticle/poly (DL-lactic-co-glycolic acid)-coated stainless steel

Biomaterials. 2012 Dec;33(34):8745-56. doi: 10.1016/j.biomaterials.2012.08.010. Epub 2012 Sep 7.


Implant-associated bacterial infections are one of the most serious complications in orthopedic surgery. Treatment of these infections often requires multiple operations, device removal, long-term systemic antibiotics, and extended rehabilitation, and is frequently ineffective, leading to worse clinical outcomes and increased financial costs. In this study, we evaluated silver nanoparticle/poly(DL-lactic-co-glycolic acid) (PLGA)-coated stainless steel alloy(SNPSA) as a potential antimicrobial implant material. We found that SNPSA exhibited strong antibacterial activity in vitro and ex vivo, and promoted MC3T3-E1 pre-osteoblasts proliferation and maturation in vitro. Furthermore, SNPSA implants induced osteogenesis while suppressing bacterial survival in contaminated rat femoral canals. Our results indicate that SNPSA has simultaneous antimicrobial and osteoinductive properties that make it a promising therapeutic material in orthopedic surgery.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Bacteria / drug effects
  • Bacterial Infections / drug therapy
  • Bacterial Infections / etiology
  • Coated Materials, Biocompatible / chemistry
  • Coated Materials, Biocompatible / pharmacology*
  • Femur / diagnostic imaging
  • Femur / growth & development
  • Femur / microbiology
  • Lactic Acid / chemistry
  • Male
  • Mice
  • Nanoparticles / chemistry
  • Osteoblasts / cytology
  • Osteogenesis*
  • Polyglycolic Acid / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Prostheses and Implants / adverse effects
  • Radiography
  • Rats
  • Rats, Sprague-Dawley
  • Silver / chemistry
  • Silver / pharmacology*
  • Stainless Steel / chemistry


  • Anti-Bacterial Agents
  • Coated Materials, Biocompatible
  • Stainless Steel
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • Silver