Dietary exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans via fish consumption and dioxin-like activity in fish determined by H4IIE-luc bioassay

Sci Total Environ. 2013 Oct 1;463-464:1192-200. doi: 10.1016/j.scitotenv.2012.07.099. Epub 2012 Sep 5.

Abstract

Dietary exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) via fish consumption in two major electronic (e) waste sites: Guiyu (GY), Guangdong Province and Taizhou (TZ), Zhejiang Province, and dioxin-like activity in fish determined by H4IIE-luc bioassay. In the present study, all fish were below EU's maximum allowable concentration in muscle of fish (4 pg WHO-TEQ/g wet wt), except crucian (4.28 pg WHO-TEQ/g wet wt) and silver carps (7.49 pg WHO-TEQ/g wet wt) collected from GY rivers. Moreover, the residual concentration in bighead carp collected from GY (2.15 pg WHO-TEQ/g wet wt) was close to the EU's action level (3 pg WHO-TEQ/g wet wt) which gives "early warning" to the competent authorities and operators to take measures to eliminate contamination. In addition, results indicated that the maximum human intake of PCDD/Fs via freshwater fish consumption in GY was 4.31 pg WHO-TEQ/kg bw/day, which exceeds the higher end of the tolerable daily intake recommended by the WHO, EC-SCF and JECFA (1-4, 2 and 2.3 pg WHO-TEQ/kg bw/day respectively). Furthermore, H4IIE-luc cell bioassay provides a very sensitive and cost-efficient screening tool for assessing the overall dioxin-like toxicity in the study, and is therefore valuable for high-throughput environmental monitoring studies.

Keywords: Electronic waste; Estimated daily intake; Freshwater fish; H4IIE-luc cell bioassay; PCDD/Fs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzofurans / adverse effects*
  • Benzofurans / analysis
  • Biological Assay / methods
  • Carps / metabolism
  • China
  • Dibenzofurans, Polychlorinated
  • Diet / adverse effects*
  • Dioxins / analysis*
  • Environmental Exposure / adverse effects
  • Environmental Exposure / analysis
  • Fishes / metabolism*
  • Humans
  • Maximum Allowable Concentration
  • Muscle, Skeletal / chemistry
  • Polychlorinated Dibenzodioxins / adverse effects
  • Polychlorinated Dibenzodioxins / analogs & derivatives*
  • Polychlorinated Dibenzodioxins / analysis
  • Tilapia / metabolism

Substances

  • Benzofurans
  • Dibenzofurans, Polychlorinated
  • Dioxins
  • Polychlorinated Dibenzodioxins
  • dibenzofuran