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, 9 (10), 969-72

Robust 4C-seq Data Analysis to Screen for Regulatory DNA Interactions

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Robust 4C-seq Data Analysis to Screen for Regulatory DNA Interactions

Harmen J G van de Werken et al. Nat Methods.

Abstract

Regulatory DNA elements can control the expression of distant genes via physical interactions. Here we present a cost-effective methodology and computational analysis pipeline for robust characterization of the physical organization around selected promoters and other functional elements using chromosome conformation capture combined with high-throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated with other functional genomics assays to facilitate physical characterization of gene regulation.

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