The α-ketoglutarate/Fe(II)-dependent dioxygenase VldW is responsible for the formation of validamycin B

Chembiochem. 2012 Oct 15;13(15):2209-11. doi: 10.1002/cbic.201200464. Epub 2012 Sep 7.

Abstract

From A to B: Through detailed biochemical investigations, we discovered that VldW, an α-ketoglutarate/Fe(II)-dependent dioxygenase, regioselectively hydroxylates validamycin A to validamycin B. The results provide insights into the biosynthesis of hydroxylated validamycins and could be used to control the metabolic outcomes of the validamycin pathway.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dioxygenases / metabolism*
  • Glycosyltransferases / metabolism
  • Inositol / analogs & derivatives
  • Inositol / chemistry
  • Inositol / metabolism
  • Ketoglutaric Acids / metabolism*
  • Stereoisomerism
  • Streptomyces / chemistry
  • Streptomyces / enzymology*

Substances

  • Ketoglutaric Acids
  • Inositol
  • validamycins
  • Dioxygenases
  • Glycosyltransferases
  • ValG protein, Streptomyces hygroscopicus