[Expression of epidermal fatty acid-binding protein in cross-species hepatocellular carcinoma]

Zhonghua Gan Zang Bing Za Zhi. 2012 Apr;20(4):270-4. doi: 10.3760/cma.j.issn.1007-3418.2012.04.009.
[Article in Chinese]

Abstract

Objective: To evaluate the utility of the cross-species screening strategy for investigating key molecule(s) involved in onset and progression of hepatocellular carcinoma (HCC).

Methods: HCC-related molecule data from our previous studies and in the literature were collected to establish a cross-species dataset. Tissue samples of HCC, non-HCC surrounding liver (para-HCC), and normal liver that were collected from humans, tree shrews and rats. The genes reported to have the most differential expression in HCC were verified by analyzing the mRNA and protein levels by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively.

Results: The cross-species dataset of HCC-related molecules included four genes: epidermal fatty acid-binding protein (E-FABP), liver (L)-FABP, tyrosine a-ketoglutarate transaminase (TKT), and cytokeratin (CK8). In humans, E-FABP mRNA expression was significantly higher (P less than 0.05) in HCC (0.87+/-0.14 vs. para-HCC: 0.64+/-0.12 and normal liver: 0.67+/-0.07; F=20.910). Similar results were obtained in tree shrew (HCC: 0.87 +/- 0.25 vs. para-HCC: 0.73 +/- 0.19 and normal liver: 0.68+/-0.19; F=3.807) and rat (HCC: 0.97+/-0.22 vs. para-HCC: 0.78+/-0.16 and normal liver: 0.80 +/- 0.13; F=4.482). The Western blotting analyses revealed a similar statistically significant trend.

Conclusion: The cross-species screening strategy for tumor genes may represent a feasible and convenient process of identifying key molecule(s) for human HCC. E-FABP may be a particularly crucial molecule for hepatocarcinogenesis.

Publication types

  • English Abstract
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adult
  • Aged
  • Animals
  • Carcinoma, Hepatocellular / metabolism*
  • Case-Control Studies
  • Epidermis / chemistry
  • Fatty Acid-Binding Proteins / metabolism*
  • Female
  • Humans
  • Liver / metabolism*
  • Liver Neoplasms / metabolism*
  • Male
  • Middle Aged
  • Rats
  • Tupaiidae / metabolism

Substances

  • Fatty Acid-Binding Proteins