Single-molecule visualization of the hybridization and dissociation of photoresponsive oligonucleotides and their reversible switching behavior in a DNA nanostructure

Angew Chem Int Ed Engl. 2012 Oct 15;51(42):10518-22. doi: 10.1002/anie.201205247. Epub 2012 Sep 10.


A framed photo of DNA: A pair of photoresponsive oligonucleotides containing azobenzene moieties was introduced into double-stranded DNA within the cavity of a DNA nanostructure (see scheme). The two dsDNAs, in contact at the center, were dissociated using UV irradiation and hybridized with visible light; this was directly observed using high-speed atomic force microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • Nanostructures / chemistry*
  • Nanotechnology / methods
  • Nucleic Acid Hybridization / methods
  • Oligonucleotides / chemistry*
  • Photochemistry


  • Oligonucleotides
  • DNA