Implementation of a genetically tuned neural platform in optimizing fluorescence from receptor-ligand binding interactions on microchips

Electrophoresis. 2012 Sep;33(17):2711-7. doi: 10.1002/elps.201200103.

Abstract

This paper describes the use of a genetically tuned neural network platform to optimize the fluorescence realized upon binding 5-carboxyfluorescein-D-Ala-D-Ala-D-Ala (5-FAM-(D-Ala)(3) ) (1) to the antibiotic teicoplanin from Actinoplanes teichomyceticus electrostatically attached to a microfluidic channel originally modified with 3-aminopropyltriethoxysilane. Here, three parameters: (i) the length of time teicoplanin was in the microchannel; (ii) the length of time 1 was in the microchannel, thereby, in equilibrium with teicoplanin, and; (iii) the amount of time buffer was flushed through the microchannel to wash out any unbound 1 remaining in the channel, are examined at a constant concentration of 1, with neural network methodology applied to optimize fluorescence. Optimal neural structure provided a best fit model, both for the training set (r(2) = 0.985) and testing set (r(2) = 0.967) data. Simulated results were experimentally validated demonstrating efficiency of the neural network approach and proved superior to the use of multiple linear regression and neural networks using standard back propagation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Computer Simulation
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Linear Models
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods*
  • Microscopy, Fluorescence / instrumentation*
  • Microscopy, Fluorescence / methods*
  • Models, Genetic
  • Neural Networks, Computer*
  • Oligopeptides / chemistry
  • Protein Binding
  • Reproducibility of Results
  • Static Electricity
  • Teicoplanin / chemistry
  • Teicoplanin / metabolism

Substances

  • Fluorescent Dyes
  • Oligopeptides
  • carboxyfluorescein-D-Ala-D-Ala-D-Ala
  • Teicoplanin