Application of Whey Protein Micro-Bead Coatings for Enhanced Strength and Probiotic Protection During Fruit Juice Storage and Gastric Incubation

J Microencapsul. 2012;29(8):713-28. doi: 10.3109/02652048.2011.638994. Epub 2012 Sep 12.


Context: Coated whey protein micro-beads may improve probiotic protection and provide delayed cell-release mechanisms.

Objective: Lactobacillus rhamnosus GG was encapsulated in whey protein micro-beads by droplet extrusion with coating via electrostatic deposition: primary-polysaccharide and secondary-whey protein.

Materials and methods: Storage studies were performed in cranberry and pomegranate juice (pH 2.4; 28 days; 4 and 25°C) followed by simulated ex vivo porcine gastric (pH 1.6) and intestinal (pH 6.6) digestion.

Results and discussion: After storage and simulated gastro-intestinal digestion, free cells, cells suspended in protein and cells encapsulated in alginate micro-beads, illustrated complete probiotic mortality, while coated micro-beads enhanced probiotic viability after juice storage (8.6 ± 0.1 log(10)CFUmL(-1)). Beads also showed significant binding of hydrophobic molecules. Coated micro-beads illustrated high gastric survival (9.5 ± 0.1 log(10)CFUmL(-1)) with 30 min delayed intestinal release relative to non-coated micro-beads.

Conclusions: Micro-bead coatings could be applied in delayed cell-release for targeted intestinal probiotic delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Beverages*
  • Digestion
  • Drug Compounding*
  • Electrophoresis, Polyacrylamide Gel
  • Flow Cytometry
  • Food Preservation*
  • Fruit*
  • Gastric Mucosa / metabolism*
  • Milk Proteins*
  • Probiotics*
  • Spectroscopy, Fourier Transform Infrared
  • Swine
  • Whey Proteins


  • Milk Proteins
  • Whey Proteins