Contrasting effects of α-tocopheryl succinate on cisplatin- and etoposide-induced apoptosis

Abstract

Targeting mitochondria is a promising strategy in tumor cell elimination. d-α-tocopheryl succinate (α-TOS), a redox-silent analog of vitamin E, is a potentially powerful tool for fighting tumors by directly affecting mitochondria. However, when used at low concentrations it can suppress apoptosis induced by the conventionally used anticancer drug cisplatin. In cells treated with cisplatin, 30μM α-TOS prominently attenuated the manifestation of characteristic features of apoptosis - release of cytochrome c from mitochondria, caspase-3-like activity, and cleavage of poly(ADP-ribose) polymerase. In contrast, cell death induced by etoposide was not inhibited but rather stimulated by α-TOS. Thus, co-treatment with α-TOS and conventional antitumor drugs should be carried out with caution.

Keywords: Apoptosis; CCCP; Cancer; Cisplatin; Etoposide; MPT; Mitochondria; OMM; PARP; carbonyl cyanide m-chlorophenylhydrazone; d-α-tocopheryl succinate; mitochondrial permeability transition; outer mitochondrial membrane; poly(ADP-ribose) polymerase; α-TOS; α-Tocopheryl succinate.