First molecular characterization of Giardia duodenalis from goats in Malaysia

Mol Cell Probes. 2013 Feb;27(1):28-31. doi: 10.1016/j.mcp.2012.08.006. Epub 2012 Aug 27.


In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the 'zoonotic' assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Protozoan / genetics*
  • Feces / parasitology*
  • Genotype
  • Giardia lamblia / classification
  • Giardia lamblia / genetics*
  • Giardia lamblia / isolation & purification
  • Giardiasis / parasitology
  • Giardiasis / veterinary*
  • Goat Diseases / parasitology*
  • Goats / parasitology
  • Malaysia
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Analysis, DNA


  • DNA, Protozoan

Associated data

  • GENBANK/HQ283227
  • GENBANK/HQ283232