Cardiomyocytes derived from human induced pluripotent stem cells as models for normal and diseased cardiac electrophysiology and contractility

Prog Biophys Mol Biol. Oct-Nov 2012;110(2-3):166-77. doi: 10.1016/j.pbiomolbio.2012.07.013. Epub 2012 Aug 7.

Abstract

Since the first description of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), these cells have garnered tremendous interest for their potential use in patient-specific analysis and therapy. Additionally, hiPSC-CMs can be derived from donor cells from patients with specific cardiac disorders, enabling in vitro human disease models for mechanistic study and therapeutic drug assessment. However, a full understanding of their electrophysiological and contractile function is necessary before this potential can be realized. Here, we review this emerging field from a functional perspective, with particular emphasis on beating rate, action potential, ionic currents, multicellular conduction, calcium handling and contraction. We further review extant hiPSC-CM disease models that recapitulate genetic myocardial disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Electrophysiological Phenomena*
  • Heart / physiology
  • Heart / physiopathology
  • Heart Diseases / pathology*
  • Heart Diseases / physiopathology*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / pathology
  • Lactic Acid
  • Myocardial Contraction*
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / pathology

Substances

  • biolactin
  • Lactic Acid