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. 2012;914:25-45.
doi: 10.1007/978-1-62703-023-6_3.

Solution NMR Studies of Integral Polytopic α-Helical Membrane Proteins: The Structure Determination of the Seven-Helix Transmembrane Receptor Sensory Rhodopsin II, pSRII

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Solution NMR Studies of Integral Polytopic α-Helical Membrane Proteins: The Structure Determination of the Seven-Helix Transmembrane Receptor Sensory Rhodopsin II, pSRII

Antoine Gautier et al. Methods Mol Biol. .

Abstract

About 30% of the proteins encoded in the genome are expressed as membrane proteins but these represent <1% of all the structures solved today. In view of the physiological and pharmaceutical significance of membrane proteins it is clear that a better and more comprehensive understanding of their three-dimensional (3D) structures at atomic resolution is required. α-Helical integral membrane proteins are generally more difficult to work with than β-barrel-type proteins and this has particularly been true for the polytopic members such as the large family of seven-helical proteins. In this chapter we describe the practical aspects of the solution-state NMR spectroscopy structure determination of the seven-helical transmembrane (7-TM) protein receptor sensory rhodopsin pSRII from the haloalkaliphilic archaeon Natronomonas pharaonis reconstituted in detergent micelles. This is the first time that a three-dimensional structure of a 7-TM protein has been determined by NMR.

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