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, 2 (5), 837-841

Investigation of the Protective Effect of Erythropoietin on Spinal Cord Injury in Rats

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Investigation of the Protective Effect of Erythropoietin on Spinal Cord Injury in Rats

Zhenghua Hong et al. Exp Ther Med.

Abstract

Erythropoietin (EPO) is a promising therapeutic agent used in a variety of spinal cord injuries. Therefore, identifying the specific molecular pathway mediating the neuronal protective effect of EPO after spinal cord injury (SCI) is of great value to the patients concerned. Platelet-derived growth factor (PDGF)-B is an important factor in the recovery of neurological function. We explored changes in the expression of PDGF-B in spinal cord injury rats after receiving EPO treatment. We used a weight-drop contusion SCI model, and EPO treatment group rats received single doses of EPO (1,000 U/kg i.p.) immediately after the operation. Seven days after the operation, the results revealed a more rapid recovery as noted by the higher BBB scores, less disruption and more neuronal regeneration of the spinal cord in the EPO treatment group than that in the SCI group. PDGF-B expression also increased in the EPO treatment group compared to that in the SCI group (P<0.01). This study showed that PDGF-B plays a role in the neuronal protective effect of EPO on spinal cord injury in rats, which may help to explain the quick recovery after EPO treatment of spinal cord injury.

Figures

Figure 1.
Figure 1.
BBB scores in a sham operation control rat, a spinal cord injury rat and an EPO treatment rat 1 (A) and 7 (B) days after SCI. *P<0.01, vs. sham operation group; #P<0.01, vs. SCI group.
Figure 2.
Figure 2.
Light photomicrographs of the spinal cord with H&E staining (x400). (A) A sham operation control rat. (B) A spinal cord injury rat. (C) An EPO treatment rat.
Figure 3.
Figure 3.
PDGF-B immunohistochemical staining of the spinal cord with DAB staining (x400). (A) A sham operation control rat. (B) A spinal cord injury rat. (C) An EPO treatment rat.
Figure 4.
Figure 4.
The number of PDGF-B-positive cells in the spinal cord from each group of rats. *P<0.01 vs. operation group; #P<0.01 vs. SCI group

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