Effect of modulating macrophage phenotype on peripheral nerve repair

Biomaterials. 2012 Dec;33(34):8793-801. doi: 10.1016/j.biomaterials.2012.08.050. Epub 2012 Sep 12.


Peripheral nerve repair across long gaps remains clinically challenging despite progress made with autograft transplantation. While scaffolds that present trophic factors and extracellular matrix molecules have been designed, matching the performance of autograft-induced repair has been challenging. In this study, we explored the effect of cytokine mediated 'biasing' of macrophage phenotypes on Schwann cell (SC) migration and axonal regeneration in vitro and in vivo. Macrophage phenotype was successfully modulated by local delivery of either Interferon-gamma (IFN-γ) or Interleukin-4 (IL-4) within polymeric nerve guidance channels, polarizing them toward pro-inflammatory (M1) or pro-healing (M2a and M2c) phenotypes, respectively. The initial polarization of macrophages to M2a and M2c phenotype results in enhanced SC infiltration and substantially faster axonal growth in a critically-sized rat sciatic nerve gap model (15 mm). The ratio of pro-healing to pro-inflammatory population of macrophages (CD206+/CCR7+), defined as regenerative bias, demonstrates a linear relationship with the number of axons at the distal end of the nerve scaffolds. The present results clearly suggest that rather than the extent of macrophage presence, their specific phenotype at the site of injury regulates the regenerative outcomes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Line
  • Cell Movement
  • Cell Proliferation
  • Immunologic Factors / therapeutic use*
  • Interferon-gamma / therapeutic use*
  • Interleukin-4 / therapeutic use*
  • Lectins, C-Type / immunology
  • Macrophages / cytology
  • Macrophages / immunology*
  • Male
  • Mannose Receptor
  • Mannose-Binding Lectins / immunology
  • Nerve Regeneration*
  • Peripheral Nerves / cytology
  • Peripheral Nerves / immunology*
  • Peripheral Nerves / physiology*
  • Phenotype
  • Rats
  • Rats, Inbred Lew
  • Receptors, CCR7 / immunology
  • Receptors, Cell Surface / immunology
  • Schwann Cells / cytology
  • Schwann Cells / immunology


  • Immunologic Factors
  • Lectins, C-Type
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Receptors, CCR7
  • Receptors, Cell Surface
  • Interleukin-4
  • Interferon-gamma