Comparison of endothelialization and neointimal formation with stents coated with antibodies against CD34 and vascular endothelial-cadherin

Biomaterials. 2012 Dec;33(35):8917-27. doi: 10.1016/j.biomaterials.2012.08.066. Epub 2012 Sep 14.


Vascular endothelial-cadherin (VE-cadherin) is exclusively expressed on the late endothelial progenitor cells (EPC). Therefore, VE-cadherin could be an ideal target surface molecule to capture circulating late EPC. In the present study, we evaluated whether anti-VE-cadherin antibody-coated stents (VE-cad stents) might accelerate endothelial recovery and reduce neointimal formation more than anti-CD34 antibody-coated stents (CD34 stents) through the superior ability to capture the late EPC. The stainless steel stents were coated with anti-human VE-cadherin antibodies or anti-human CD34 antibodies under the same condition. In vitro, VE-cad stents showed higher number of adhering EPC (823.6 ± 182.2 versus 379.2 ± 137.2 cells per HPF, p < 0.001). VE-cad stents also demonstrated better specific capturing of cells with endothelial lineage markers than CD34 stents did in flow cytometric analysis. VE-cad stents showed more effective re-endothelialization after 1 h, 24 h, and 3 days in vivo. At 42 days, VE-cad stents demonstrated significantly smaller neointima area (0.92 ± 0.38 versus 1.24 ± 0.41 mm(2), p = 0.002) and significantly lower PCNA positive cells in neointima (1684.8 ± 658.8/mm(2) versus 2681.7 ± 375.1/mm(2), p = 0.008), compared with CD34 stents. In conclusion, VE-cad stents captured EPC and endothelial cells more selectively in vitro, accelerated re-endothelialization over stents, and reduced neointimal formation in vivo, compared with CD34 stents.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / chemistry*
  • Antigens, CD / chemistry*
  • Antigens, CD34 / chemistry*
  • Cadherins / chemistry*
  • Cell Proliferation
  • Coated Materials, Biocompatible
  • Endothelial Cells / cytology
  • Endothelium / metabolism
  • Humans
  • Leukocytes, Mononuclear / chemistry
  • Neointima / metabolism*
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Stents*


  • Antibodies
  • Antigens, CD
  • Antigens, CD34
  • Cadherins
  • Coated Materials, Biocompatible
  • cadherin 5