Anti-inflammatory effects of naringin in chronic pulmonary neutrophilic inflammation in cigarette smoke-exposed rats

J Med Food. 2012 Oct;15(10):894-900. doi: 10.1089/jmf.2012.2251. Epub 2012 Sep 17.


Naringin, a well-known flavanone glycoside of grapefruit and citrus fruits, was found to be as an effective anti-inflammatory compound in our previous lipopolysaccharide-induced acute lung injury mouse model via blockading activity of nuclear factor κB. The current study sought to explore the anti-inflammatory effects of naringin on chronic pulmonary neutrophilic inflammation in cigarette smoke (CS)-induced rats. Seventy Sprague-Dawley rats were randomly divided into seven groups to study the effects of CS with or without various concentrations of naringin or saline for 8 weeks. The results revealed that naringin supplementation at 20, 40, and 80 mg/kg significantly increased body weight of CS-induced rats as compared to that in the CS group. Moreover, naringin of 20, 40, and 80 mg/kg prevented CS-induced infiltration of neutrophils and activation of myeloperoxidase and matrix metalloproteinase-9, in parallel with suppression of the release of cytokines, such as tumor necrosis factor-α and interleukin-8 (IL-8). IL-10 in bronchoalveolar lavage fluid was significantly suppressed after CS exposure, but dose dependently elevated by naringin. The results from hematoxylin and eosin staining revealed that naringin dose dependently reduced CS-induced infiltration of inflammatory cells, thickening of the bronchial wall, and expansion of average alveolar airspace. In conclusion, our data suggest that naringin is an effective anti-inflammatory compound for attenuating chronic pulmonary neutrophilic inflammation in CS-induced rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Female
  • Flavanones / pharmacology*
  • Interleukin-10 / antagonists & inhibitors
  • Interleukin-10 / metabolism
  • Interleukin-8 / antagonists & inhibitors
  • Interleukin-8 / metabolism
  • Male
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinase 9 / metabolism
  • Neutrophils / drug effects*
  • Neutrophils / pathology
  • Peroxidase / genetics
  • Peroxidase / metabolism
  • Pneumonia / drug therapy*
  • Pneumonia / pathology
  • Pulmonary Disease, Chronic Obstructive / drug therapy
  • Pulmonary Disease, Chronic Obstructive / pathology
  • Rats
  • Rats, Sprague-Dawley
  • Smoking / adverse effects*
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors
  • Tumor Necrosis Factor-alpha / metabolism


  • Anti-Inflammatory Agents
  • Flavanones
  • Interleukin-8
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Peroxidase
  • Matrix Metalloproteinase 9
  • Mmp9 protein, rat
  • naringin