Abstract
The discovery of a novel canine norovirus was recently reported. The VP1 capsid protein of this canine norovirus was expressed in Saccharomyces cerevisiae and purified with a yield of 1.6-1.8 mg/L. The yeast-expressed VP1 protein self-assembled into virus-like particles and exhibited antigenicity, proving to be suitable for serological assays.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caliciviridae Infections / diagnosis
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Caliciviridae Infections / veterinary
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Caliciviridae Infections / virology
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Capsid Proteins / biosynthesis*
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Capsid Proteins / genetics
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Capsid Proteins / isolation & purification*
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Dog Diseases / diagnosis
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Dog Diseases / virology
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Dogs
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Gene Expression*
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Norovirus / genetics*
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Norovirus / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism