Production and purification of theVP1 capsid protein of a novel canine norovirus using the Saccharomyces cerevisiae expression system

J Microbiol Methods. 2012 Dec;91(3):358-60. doi: 10.1016/j.mimet.2012.09.007. Epub 2012 Sep 15.

Abstract

The discovery of a novel canine norovirus was recently reported. The VP1 capsid protein of this canine norovirus was expressed in Saccharomyces cerevisiae and purified with a yield of 1.6-1.8 mg/L. The yeast-expressed VP1 protein self-assembled into virus-like particles and exhibited antigenicity, proving to be suitable for serological assays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caliciviridae Infections / diagnosis
  • Caliciviridae Infections / veterinary
  • Caliciviridae Infections / virology
  • Capsid Proteins / biosynthesis*
  • Capsid Proteins / genetics
  • Capsid Proteins / isolation & purification*
  • Dog Diseases / diagnosis
  • Dog Diseases / virology
  • Dogs
  • Gene Expression*
  • Norovirus / genetics*
  • Norovirus / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Capsid Proteins