Human pluripotent stem cell-derived mesenchymal stem cells prevent allergic airway inflammation in mice

Stem Cells. 2012 Dec;30(12):2692-9. doi: 10.1002/stem.1241.


We previously found that mesenchymal stem cells (MSCs) derived from human-induced pluripotent stem cells (iPSCs) exerted immunomodulatory effects on Th2-mediated allergic rhinitis in vitro. However, their contribution to the asthma and allergic rhinitis in animal models remains unclear. In this study, we developed a mouse model of ovalbumin (OVA)-induced allergic inflammation in both the upper and lower airways and evaluated the effects of the systemic administration of human iPSC-MSCs and bone marrow-derived MSCs (BM-MSCs) on allergic inflammation. Our results showed that treatments with both the iPSC-MSCs and BM-MSCs before the challenge phase protected the animals from the majority of allergy-specific pathological changes. This protection included an inhibition of inflammatory cell infiltration and mucus production in the lung, a reduction in eosinophil infiltration in the nose, and a decrease in inflammatory cell infiltration in both the bronchoalveolar and nasal lavage fluids. In addition, treatment with iPSC-MSCs or BM-MSCs before the challenge phase resulted in reduced serum levels of Th2 immunoglobulins (e.g., IgE) and decreased levels of Th2 cytokines including interleukin (IL)-4, IL-5, or IL-13 in the bronchoalveolar and/or nasal lavage fluids. Similar therapeutic effects were observed when the animals were pretreated with human iPSC-MSCs before the sensitization phase. These data suggest that iPSC-MSCs may be used as an alternative strategy to adult MSCs in the treatment of asthma and allergic rhinitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asthma / immunology
  • Asthma / surgery
  • Asthma / therapy*
  • Bronchial Hyperreactivity / immunology
  • Bronchial Hyperreactivity / surgery
  • Bronchial Hyperreactivity / therapy*
  • Cytokines / biosynthesis
  • Cytokines / immunology
  • Disease Models, Animal
  • Eosinophils / immunology
  • Female
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Immunotherapy, Adoptive
  • Mesenchymal Stem Cell Transplantation / methods*
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Nasal Cavity / immunology
  • Pluripotent Stem Cells / cytology
  • Pluripotent Stem Cells / immunology*
  • Pluripotent Stem Cells / transplantation*
  • Th2 Cells / immunology


  • Cytokines
  • Immunoglobulin E