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. 2013:923:321-33.
doi: 10.1007/978-1-62703-026-7_23.

Genome-wide chromatin immunoprecipitation-sequencing in Plasmodium

Jose-Juan Lopez-Rubio  1 , T Nicolai Siegel, Artur Scherf
Affiliations

Genome-wide chromatin immunoprecipitation-sequencing in Plasmodium

Jose-Juan Lopez-Rubio et al. Methods Mol Biol. 2013.

Abstract

Chromatin immunoprecipitation (ChIP) studies have been used extensively in recent years to study the functional role of histone marks, variant histones, and other chromatin factors in gene expression in the human malaria parasite, Plasmodium falciparum. In this chapter, we present a ChIP-sequencing protocol optimized for blood-stage forms of this parasite. The processing of the immunoprecipitated DNA prior to high-throughput sequencing is performed in a way to minimize amplification biases due to the high genomic AT-content of the parasite.

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Figures

Fig. 1
Fig. 1
Two-step genome-wide ChIP protocol. DNA fragment library preparation is needed for microarray or high-throughput sequencing.
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