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, 2012, 759407

A Systems Approach and Skeletal Myogenesis

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A Systems Approach and Skeletal Myogenesis

Yoshiaki Ito et al. Comp Funct Genomics.

Abstract

Skeletal myogenesis depends on the strict regulation of the expression of various gene subsets. Therefore, the understanding of genome wide gene regulation is imperative for elucidation of skeletal myogenesis. In recent years, systems approach has contributed to the understanding of various biological processes. Our group recently revealed the critical genome network of skeletal myogenesis by using a novel systems approach combined with whole-mount in situ hybridization (WISH) database, high-throughput screening, and microarray analysis. In this paper, we introduce our systems approach for understanding the myogenesis regulatory network and describe the advantages of systems approach.

Figures

Figure 1
Figure 1
Scheme of our systems approach. WISH database EMBRYS identified a novel myogenesis regulator RP58. High-throughput transfection assay and microarray analysis identified upstream and downstream factors of RP58. This multicombined approach is useful for elucidation of molecular network in the developmental process.
Figure 2
Figure 2
Proposed myogenesis regulatory network by our systems approach in myoblasts; Id proteins are expressed and inhibit the myogenic bHLH factors. During myogenic differentiation, RP58 is promoted by MyoD and represses the Id transcription. Muscle specific genes are then activated by myogenic bHLH factors.

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