Novel human brain cDNA encoding a 34,000 Mr protein n-chimaerin, related to both the regulatory domain of protein kinase C and BCR, the product of the breakpoint cluster region gene

J Mol Biol. 1990 Jan 5;211(1):11-6. doi: 10.1016/0022-2836(90)90006-8.

Abstract

A novel human brain complementary DNA sequence encodes n-chimaerin, a 34,000 Mr protein. A single cysteine-rich sequence CX2CX13CX2CX7CX7C in the N-terminal half of n-chimaerin shares almost 50% identity with corresponding sequences in the C1 regulatory domain of protein kinase C. The C-terminal half of n-chimaerin has 42% identity with the C-terminal region (amino acid residues 1050 to 1225) of BCR, the product of the breakpoint cluster region gene involved in Philadelphia (Ph') chromosome translocation. n-Chimaerin mRNA (2.2 x 10(3) base-pairs) is specifically expressed in the brain, with the highest amounts being in the hippocampus and cerebral cortex. The mRNA has a neuronal distribution and is expressed in neuroblastoma cells, but not in C6 glioma or primary astrocyte cultures. The similarity of two separate regions of n-chimaerin to domains of protein kinase C and BCR has intriguing implications with respect to its evolutionary origins, its function in the brain and potential phorbol-ester-binding properties.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Brain / metabolism
  • Chimerin 1
  • DNA / genetics*
  • Gene Expression
  • Genes
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Multigene Family*
  • Nerve Tissue Proteins / genetics*
  • Philadelphia Chromosome*
  • Protein Kinase C / genetics*
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Chimerin 1
  • Nerve Tissue Proteins
  • RNA, Messenger
  • DNA
  • Protein Kinase C

Associated data

  • GENBANK/X51408