Background. Sedentary behavior is emerging as an important risk factor for poor health. Physical activity has proven to be important in determining overall successful aging (SA) among older adults; however, no data exists on the influence of sedentary behavior on SA. The purpose of this analysis was to determine whether there is an association between sedentary behavior and successful aging, independent of physical activity levels. Methods. 9,478 older (M = 4,245; F = 5,233) and 10,060 middle-aged (M = 4.621; F = 5,439) adults from the Healthy Aging cycle of the Canadian Community Health Survey were analyzed. Multivariate logistic regressions were conducted with SA and its three components as outcomes while physical activity and sedentary behavior were entered as main exposures. Results. Among older adults, compared to those who were sedentary (4 hours or more/day), those who were moderately (2-4 hours/day) and least sedentary (<2 hours/day) were 38% (OR: 1.38; CI: 1.12-1.69) and 43% (OR: 1.43; CI: 1.23-1.67) more likely to age successfully, respectively. Among middle-aged adults, those who were least sedentary were 43% (OR: 1.43; CI: 1.25-1.63) more likely to age successfully. Conclusions. These novel findings suggest that sedentary activities are significantly associated with lower odds of SA among middle-aged and older adults, potentially in a dose-dependent manner.