Salivary microRNAs and Oral Cancer Detection

Methods Mol Biol. 2013;936:313-24. doi: 10.1007/978-1-62703-083-0_24.

Abstract

MicroRNAs (miRNAs) in human saliva have recently become an emerging field in saliva research for -diagnostics applications and its potential role in biological implications. miRNAs are short noncoding RNA molecules that play important roles in regulating a variety of cellular processes. Dysregulation of miRNAs are known to be associated with many diseases. miRNAs were found present in the saliva of OSCC patients and could serve as potential biomarkers for oral cancer detection. Understanding the biological function of miRNAs in association with diseases is important towards utilizing miRNAs as diagnostic markers. There are currently a variety of profiling methods available for detecting miRNA expression levels. In this chapter, we overview the Applied Biosystem Stem-loop RT based Taqman MicroRNA Assay for salivary miRNA profiling. Using this highly sensitive and specific assay, miRNAs in saliva are profiled with only a few nanograms of starting RNA. This method is also applicable for studying biomarkers in other body fluids or clinical samples that contain small amounts of RNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biomarkers, Tumor / genetics*
  • Computational Biology / methods
  • Gene Expression Profiling / methods*
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / isolation & purification
  • MicroRNAs / metabolism*
  • Mouth Neoplasms / diagnosis*
  • Mouth Neoplasms / genetics*
  • Real-Time Polymerase Chain Reaction / instrumentation
  • Real-Time Polymerase Chain Reaction / methods
  • Saliva / metabolism*

Substances

  • Biomarkers, Tumor
  • MicroRNAs