Sulfonyl fluoride analogues as activity-based probes for serine proteases

Chembiochem. 2012 Nov 5;13(16):2327-30. doi: 10.1002/cbic.201200531. Epub 2012 Sep 24.


Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Molecular Probes / chemistry
  • Molecular Probes / pharmacology*
  • Molecular Structure
  • Serine Proteases / metabolism*
  • Serine Proteinase Inhibitors / chemistry
  • Serine Proteinase Inhibitors / pharmacology*
  • Structure-Activity Relationship
  • Sulfinic Acids / chemistry
  • Sulfinic Acids / pharmacology*


  • Molecular Probes
  • Serine Proteinase Inhibitors
  • Sulfinic Acids
  • sulfuryl fluoride
  • Serine Proteases