Characterization of aroma compounds in Chinese bayberry (Myrica rubra Sieb. et Zucc.) by gas chromatography mass spectrometry (GC-MS) and olfactometry (GC-O)

J Food Sci. 2012 Oct;77(10):C1030-5. doi: 10.1111/j.1750-3841.2012.02747.x. Epub 2012 Sep 25.


The aroma-active compounds in Chinese bayberry were identified using gas chromatography-olfactometry (GC-O) and GC-mass spectrometry techniques. The volatile compounds were extracted using Liquid-liquid extraction, solvent-assisted flavor evaporation and headspace solid-phase microextraction (HS-SPME), respectively. On the basis of odor intensity, the most important aroma compounds in Chinese bayberry samples were caryophyllene, menthol, 4-terpineol, linalool oxide, linalool, benzyl alcohol, α-methylbenzyl alcohol, β-phenylethanol, 3-methylbutanoic acid and acetic acid, and so on. Moreover, HS-SPME technique was employed to investigate the aroma compounds among immature and mature waxberry fruits. The results showed that terpenes (for example, β-caryophyllene) was predominant and its concentration represented over 89.9% of the overall compounds, and alcohols, aldehydes, ketones, esters, acids, and others were typically present in lesser amounts. Finally, principal component analysis revealed that there was also significant difference between immature and mature waxberry fruits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclic Monoterpenes
  • Benzyl Alcohol / analysis
  • Benzyl Alcohols / analysis
  • Chromatography, Gas / methods*
  • Fruit / chemistry
  • Gas Chromatography-Mass Spectrometry / methods*
  • Liquid-Liquid Extraction / methods
  • Monoterpenes / analysis
  • Myrica / chemistry*
  • Odorants / analysis*
  • Phenylethyl Alcohol / analysis
  • Polycyclic Sesquiterpenes
  • Sesquiterpenes / analysis
  • Solid Phase Microextraction / methods
  • Solvents / chemistry
  • Taste
  • Volatile Organic Compounds / isolation & purification*


  • Acyclic Monoterpenes
  • Benzyl Alcohols
  • Monoterpenes
  • Polycyclic Sesquiterpenes
  • Sesquiterpenes
  • Solvents
  • Volatile Organic Compounds
  • caryophyllene
  • linalool
  • methylphenyl carbinol
  • Benzyl Alcohol
  • Phenylethyl Alcohol