Using gene-environment interaction analyses to clarify the role of well-done meat and heterocyclic amine exposure in the etiology of colorectal polyps

Am J Clin Nutr. 2012 Nov;96(5):1119-28. doi: 10.3945/ajcn.112.040345. Epub 2012 Sep 26.

Abstract

Background: The role of well-done meat intake and meat-derived mutagen heterocyclic amine (HCA) exposure in the risk of colorectal neoplasm has been suggested but not yet established.

Objective: With the use of gene-environment interaction analyses, we sought to clarify the association of HCA exposure with colorectal polyp risk.

Design: In a case-control study including 2057 colorectal polyp patients and 3329 controls, we evaluated 16 functional genetic variants to construct an HCA-metabolizing score. To derive dietary HCA-exposure amount, data were collected regarding dietary intake of meat by cooking method and degree of doneness.

Results: A 2-fold elevated risk associated with high red meat intake was found for colorectal polyps or adenomas in subjects with a high HCA-metabolizing risk score, whereas the risk was 1.3- to 1.4-fold among those with a low risk score (P-interaction ≤ 0.05). The interaction was stronger for the risk of advanced or multiple adenomas, in which an OR of 2.8 (95% CI: 1.8, 4.6) was observed for those with both a high HCA-risk score and high red meat intake (P-interaction = 0.01). No statistically significant interaction was found in analyses that used specific HCA exposure derived from dietary data.

Conclusion: High red meat intake is associated with an elevated risk of colorectal polyps, and this association may be synergistically modified by genetic factors involved in HCA metabolism.

Publication types

  • Multicenter Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenoma / enzymology
  • Adenoma / etiology*
  • Adenoma / genetics
  • Adult
  • Aged
  • Alleles
  • Amines / administration & dosage*
  • Amines / adverse effects*
  • Animals
  • Case-Control Studies
  • Colonic Polyps / enzymology
  • Colonic Polyps / etiology*
  • Colonic Polyps / genetics
  • Colorectal Neoplasms / enzymology
  • Colorectal Neoplasms / etiology*
  • Colorectal Neoplasms / genetics
  • DNA, Neoplasm / chemistry
  • DNA, Neoplasm / genetics
  • Female
  • Gene-Environment Interaction*
  • Humans
  • Male
  • Meat / adverse effects*
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • Risk Factors

Substances

  • Amines
  • DNA, Neoplasm