Inhibition of Rho-kinase attenuates endothelial-leukocyte interaction during ischemia-reperfusion injury

Vasc Med. 2012 Dec;17(6):379-85. doi: 10.1177/1358863X12459790. Epub 2012 Sep 26.

Abstract

Resuscitation from hemorrhagic shock induces endothelial dysfunction and activates inflammatory cascades leading to organ damage. Following restoration of blood flow to ischemic vascular beds, leukocyte-endothelium interactions leading to leukocyte infiltration into the vascular wall occur very early due, in part, to the loss of endothelium-derived nitric oxide (NO). The mechanism by which ischemia-reperfusion injury impairs endothelium-derived NO is not completely understood. We hypothesized that inhibition of Rho-kinase could exert beneficial effects following hemorrhagic shock by preserving endothelial function and attenuating leukocyte trafficking in the microcirculation. Using intravital microscopy, we found that resuscitation from hemorrhage acutely increased the number of rolling and adherent leukocytes in the mouse splanchnic microcirculation. Treatment of mice with the Rho-kinase inhibitor fasudil, markedly attenuated leukocyte-endothelium interaction in response to hemorrhage/reinfusion. The beneficial effect of fasudil was not observed in endothelial nitric oxide synthase (eNOS)(-/-) mice. In conclusion, inhibition of Rho-kinase prevents inflammatory leukocyte trafficking in the microcirculation via an eNOS-dependent mechanism. Our data support a role for Rho-kinase inhibitors in the treatment of ischemia-reperfusion injury.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / analogs & derivatives*
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology
  • Animals
  • Endothelium, Vascular / drug effects*
  • Endothelium, Vascular / enzymology
  • Leukocytes / drug effects*
  • Leukocytes / enzymology
  • Mice
  • Mice, Inbred C3H
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type III / metabolism
  • Protein Kinase Inhibitors / pharmacology*
  • Reperfusion Injury / drug therapy
  • Reperfusion Injury / enzymology*
  • Reperfusion Injury / physiopathology
  • rho-Associated Kinases / antagonists & inhibitors*

Substances

  • Protein Kinase Inhibitors
  • Nitric Oxide
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Nitric Oxide Synthase Type III
  • rho-Associated Kinases
  • fasudil