Electron microscopical demonstration of glucose-6-phosphatase in native cryostat sections fixed with glutaraldehyde through semipermeable membranes

Histochemistry. 1979;65(1):41-7. doi: 10.1007/BF00496684.

Abstract

The cytochemical demonstration of glucose-6-phosphatase (G6Pase) activity in native cryostat sections fixed with glutaraldehyde through semipermeable membranes is superior to conventional methods with regard to exact localization and lack of inactivation and diffusion of the enzyme, together with simultaneous excellent preservation of the tissue fine structure. In rat liver not only hepatocytes but also many bile duct epithelia and endothelia of arterioles and venules show a marked G6Pase activity in the membranes of the endoplasmic reticulum including the nuclear envelope.

MeSH terms

  • Animals
  • Bile Ducts, Intrahepatic / enzymology
  • Endoplasmic Reticulum / enzymology
  • Fixatives
  • Frozen Sections
  • Glucose-6-Phosphatase / analysis*
  • Glutaral
  • Histocytochemistry / methods
  • Intracellular Membranes / enzymology
  • Liver / enzymology*
  • Liver / ultrastructure
  • Membranes
  • Microscopy, Electron
  • Rats

Substances

  • Fixatives
  • Glucose-6-Phosphatase
  • Glutaral