The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model

Clinics (Sao Paulo). 2012 Sep;67(9):1077-85. doi: 10.6061/clinics/2012(09)16.

Abstract

Objective: Osteoporosis increases the risk of bone fractures and may impair fracture healing. The aim of this study was to investigate whether alpha-tocopherol can improve the late-phase fracture healing of osteoporotic bones in ovariectomized rats.

Method: In total, 24 female Sprague-Dawley rats were divided into three groups. The first group was sham-operated, and the other two groups were ovariectomized. After two months, the right femora of the rats were fractured under anesthesia and internally repaired with K-wires. The sham-operated and ovariectomized control rat groups were administered olive oil (a vehicle), whereas 60 mg/kg of alpha-tocopherol was administered via oral gavage to the alpha-tocopherol group for six days per week over the course of 8 weeks. The rats were sacrificed, and the femora were dissected out. Computed tomography scans and X-rays were performed to assess fracture healing and callus staging, followed by the assessment of callus strengths through the biomechanical testing of the bones.

Results: Significantly higher callus volume and callus staging were observed in the ovariectomized control group compared with the sham-operated and alpha-tocopherol groups. The ovariectomized control group also had significantly lower fracture healing scores than the sham-operated group. There were no differences between the alpha-tocopherol and sham-operated groups with respect to the above parameters. The healed femora of the ovariectomized control group demonstrated significantly lower load and strain parameters than the healed femora of the sham-operated group. Alpha-tocopherol supplementation was not able to restore these biomechanical properties.

Conclusion: Alpha-tocopherol supplementation appeared to promote bone fracture healing in osteoporotic rats but failed to restore the strength of the fractured bone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Biomechanical Phenomena
  • Bone Density
  • Disease Models, Animal
  • Female
  • Femur / diagnostic imaging
  • Femur / drug effects
  • Fracture Healing / drug effects*
  • Fractures, Bone / drug therapy*
  • Humans
  • Osteoporosis, Postmenopausal* / diagnostic imaging
  • Ovariectomy
  • Pliability
  • Radiography
  • Rats
  • Rats, Sprague-Dawley
  • Tensile Strength
  • Time Factors
  • Tomography Scanners, X-Ray Computed
  • alpha-Tocopherol / pharmacology*

Substances

  • Antioxidants
  • alpha-Tocopherol