Identification of avocado (Persea americana) pulp proteins by nano-LC-MS/MS via combinatorial peptide ligand libraries

Electrophoresis. 2012 Sep;33(18):2799-805. doi: 10.1002/elps.201200254.

Abstract

Avocado (Persea americana) proteins have been scarcely studied despite their importance, especially in food related allergies. The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries at pH 7.4 and under conditions mimicking reverse-phase capture at pH 2.2. The total number of unique gene products identified amounts to 1012 proteins, of which 174 are in common with the control, untreated sample, 190 are present only in the control and 648 represent the new species detected via combinatorial peptide ligand libraries of all combined eluates and likely represent low-abundance proteins. Among the 1012 proteins, it was possible to identify the already known avocado allergen Pers a 1 and different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin-like protein, a glucanase, and an isoflavone reductase like protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid / methods*
  • Electrophoresis, Polyacrylamide Gel
  • Nanotechnology
  • Peptide Library
  • Persea / chemistry*
  • Plant Proteins / analysis*
  • Plant Proteins / chemistry
  • Plant Proteins / classification
  • Proteome / analysis*
  • Proteomics
  • Tandem Mass Spectrometry / methods*

Substances

  • Peptide Library
  • Plant Proteins
  • Proteome