A mouse model for HIV-1 entry

Proc Natl Acad Sci U S A. 2012 Sep 25;109(39):15859-64. doi: 10.1073/pnas.1213409109. Epub 2012 Sep 10.


Passive transfer of neutralizing antibodies against HIV-1 can prevent infection in macaques and seems to delay HIV-1 rebound in humans. Anti-HIV antibodies are therefore of great interest for vaccine design. However, the basis for their in vivo activity has been difficult to evaluate systematically because of a paucity of small animal models for HIV infection. Here we report a genetically humanized mouse model that incorporates a luciferase reporter for rapid quantitation of HIV entry. An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing / immunology
  • Antibodies, Neutralizing / metabolism
  • Antibodies, Neutralizing / pharmacology*
  • Antibodies, Viral / immunology
  • Antibodies, Viral / metabolism
  • Antibodies, Viral / pharmacology*
  • Disease Models, Animal*
  • HIV Infections / genetics
  • HIV Infections / immunology
  • HIV Infections / metabolism*
  • HIV-1 / genetics
  • HIV-1 / immunology
  • HIV-1 / metabolism*
  • Humans
  • Macaca
  • Mice
  • Mice, Transgenic
  • Virus Internalization*


  • Antibodies, Neutralizing
  • Antibodies, Viral