BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis

Plant J. 2013 Feb;73(3):380-91. doi: 10.1111/tpj.12036. Epub 2012 Dec 31.

Abstract

Brassinosteroids (BRs) are essential for various aspects of plant development. Cellular BR homeostasis is critical for proper growth and development of plants; however, its regulatory mechanism remains largely unknown. BAT1 (BR-related acyltransferase 1), a gene encoding a putative acyltransferase, was found to be involved in vascular bundle development in a full-length cDNA over-expressor (FOX) screen. Over-expression of BAT1 resulted in typical BR-deficient phenotypes, which were rescued by exogenously applied castasterone and brassinolide. Analyses of BR profiles demonstrated that BAT1 alters levels of several brassinolide biosynthetic intermediates, including 6-deoxotyphasterol, typhasterol and 6-deoxocastasterone. BAT1 is mainly localized in the endoplasmic reticulum. BAT1 is highly expressed in young tissues and vascular bundles, and its expression is induced by auxin. These data suggest that BAT1 is involved in BR homeostasis, probably by conversion of brassinolide intermediates into acylated BR conjugates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyltransferases / metabolism*
  • Amino Acid Transport Systems / genetics
  • Amino Acid Transport Systems / physiology*
  • Arabidopsis / enzymology
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / physiology*
  • Brassinosteroids / metabolism*
  • DNA, Complementary
  • Endoplasmic Reticulum / metabolism
  • Homeostasis
  • Plant Growth Regulators / metabolism*
  • Plants, Genetically Modified

Substances

  • Amino Acid Transport Systems
  • Arabidopsis Proteins
  • BAT1 protein, Arabidopsis
  • Brassinosteroids
  • DNA, Complementary
  • Plant Growth Regulators
  • Acyltransferases