Cloning of a hsp70-related Gene Expressed in Mouse Spermatids

Biochem Biophys Res Commun. 1990 Jan 15;166(1):43-9. doi: 10.1016/0006-291x(90)91909-c.

Abstract

A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • DNA / genetics
  • Gene Expression
  • Gene Library
  • Genes*
  • Haploidy
  • Heat-Shock Proteins / genetics*
  • Male
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Sequence Homology, Nucleic Acid
  • Spermatids / metabolism*

Substances

  • Heat-Shock Proteins
  • DNA

Associated data

  • GENBANK/M32218