Amorphous silica nanoparticles enhance cross-presentation in murine dendritic cells

Biochem Biophys Res Commun. 2012 Oct 26;427(3):553-6. doi: 10.1016/j.bbrc.2012.09.095. Epub 2012 Sep 26.


Nanomaterials (NMs) exhibit unique physicochemical properties and innovative functions, and they are increasingly being used in a wide variety of fields. Ensuring the safety of NMs is now an urgent task. Recently, we reported that amorphous silica nanoparticles (nSPs), one of the most widely used NMs, enhance antigen-specific cellular immune responses and may therefore aggravate immune diseases. Thus, to ensure the design of safer nSPs, investigations into the effect of nSPs on antigen presentation in dendritic cells, which are central orchestrators of the adaptive immune response, are now needed. Here, we show that nSPs with diameters of 70 and 100 nm enhanced exogenous antigen entry into the cytosol from endosomes and induced cross-presentation, whereas submicron-sized silica particles (>100 nm) did not. Furthermore, we show that surface modification of nSPs suppressed cross-presentation. Although further studies are required to investigate whether surface-modified nSPs suppress immune-modulating effects in vivo, the current results indicate that appropriate regulation of the characteristics of nSPs, such as size and surface properties, will be critical for the design of safer nSPs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation / drug effects*
  • Cells, Cultured
  • Cross-Priming / drug effects*
  • Dendritic Cells / drug effects*
  • Dendritic Cells / immunology
  • Mice
  • Mice, Inbred C57BL
  • Nanoparticles / adverse effects*
  • Particle Size
  • Silicon Dioxide / adverse effects*


  • Silicon Dioxide