A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells

Biochem Biophys Res Commun. 1990 Jan 30;166(2):642-7. doi: 10.1016/0006-291x(90)90857-j.


When HeLa cells were exposed to heat shock or other stresses, a 40-kDa protein (p40) was induced in addition to the classical heat shock proteins with molecular sizes of 110-, 90-, 70-, and 47-kDa. The p40 was induced not only by heat shock but by arsenite and 2-azetidine carboxylic acid. Also, it was induced in rat, mouse and chick cells by these stresses. The p40 was a basic protein (pI divided by 9.2) as judged by two-dimensional gel electrophoresis. To our knowledge, p40 is a novel heat shock protein in mammalian and avian cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arsenic / pharmacology
  • Arsenites*
  • Cells, Cultured
  • Chick Embryo
  • Dactinomycin / pharmacology
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression Regulation / drug effects
  • HeLa Cells
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism*
  • Isoelectric Point
  • Mice
  • Molecular Weight
  • Rats


  • Arsenites
  • Heat-Shock Proteins
  • Dactinomycin
  • arsenite
  • Arsenic