Isolation and characterization of canine amniotic membrane-derived multipotent stem cells

PLoS One. 2012;7(9):e44693. doi: 10.1371/journal.pone.0044693. Epub 2012 Sep 14.


Recent studies have shown that amniotic membrane tissue is a rich source of stem cells in humans. In clinical applications, the amniotic membrane tissue had therapeutic effects on wound healing and corneal surface reconstruction. Here, we successfully isolated and identified multipotent stem cells (MSCs) from canine amniotic membrane tissue. We cultured the canine amniotic membrane-derived multipotent stem cells (cAM-MSCs) in low glucose DMEM medium. cAM-MSCs have a fibroblast-like shape and adhere to tissue culture plastic. We characterized the immunophenotype of cAM-MSCs by flow cytometry and measured cell proliferation by the cumulative population doubling level (CPDL). We performed differentiation studies for the detection of trilineage multipotent ability, under the appropriate culture conditions. Taken together, our results show that cAM-MSCs could be a rich source of stem cells in dogs. Furthermore, cAM-MSCs may be useful as a cell therapy application for veterinary regenerative medicine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipogenesis
  • Amnion / cytology*
  • Animals
  • Biomarkers / metabolism
  • Cell Differentiation
  • Chondrogenesis
  • Dogs
  • Flow Cytometry / methods
  • Gene Expression Profiling
  • Immunophenotyping
  • Multipotent Stem Cells / cytology*
  • Multipotent Stem Cells / metabolism*
  • Neurogenesis
  • Primary Cell Culture / methods


  • Biomarkers

Grant support

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST, 2010-0020265). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.