Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation

Cell. 1990 Feb 9;60(3):375-86. doi: 10.1016/0092-8674(90)90589-7.

Abstract

The ubiquitous Oct-1 and lymphoid Oct-2 POU homeodomain transcription factors bind to the same DNA sequence but differ in their activation potential. Oct-2 is a positive, negative, or neutral regulator of beta-globin transcription depending on the position and sequence of multimerized binding sites. To activate transcription, Oct-2 relies on two interdependent nonacidic domains, an N-terminal glutamine-rich region and a C-terminal serine-, threonine-, and proline-rich region. Oct-1 also contains a functional glutamine-rich region but fails to activate beta-globin transcription in our assay because the Oct-1 C-terminus is inactive, indicating that differential activation by Oct-1 and Oct-2 is determined by the combination of multiple activation domains. Oct-2 displays a unique phosphorylation pattern that is absent from molecules lacking one or the other activation domain, suggesting the activation domains have a role in inducing protein phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation
  • Genetic Vectors
  • Globins / genetics*
  • HeLa Cells / metabolism
  • Host Cell Factor C1
  • Humans
  • Molecular Sequence Data
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Oligonucleotide Probes
  • Phosphorylation
  • Promoter Regions, Genetic
  • Protein Biosynthesis
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Transcriptional Activation*

Substances

  • DNA-Binding Proteins
  • HCFC1 protein, human
  • Host Cell Factor C1
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Oligonucleotide Probes
  • POU2F1 protein, human
  • POU2F2 protein, human
  • Transcription Factors
  • Globins