MicroRNA-330 is an oncogenic factor in glioblastoma cells by regulating SH3GL2 gene

PLoS One. 2012;7(9):e46010. doi: 10.1371/journal.pone.0046010. Epub 2012 Sep 21.

Abstract

MicroRNAs have recently emerged as key regulators of cancers. This study was therefore conducted to investigate the role of miR-330 in biological behaviors of human glioblastoma U87 and U251 cell lines and its molecular mechanism. SH3GL2 gene was identified as the target of miR-330. MiR-330 overexpression was established by transfecting miR-330 precursor into U87 and U251 cells, and its effects on proliferation, migration, invasion, cell cycle and apoptosis were studied. Overexpression of miR-330 can enhance cellular proliferation, promote migration and invasion, activate cell cycle and also inhibit apoptosis in U87 and U251 cells. Collectively, these above-mentioned results suggest that miRNA-330 plays an oncogenic role in human glioblastoma by regulating SH3GL2 gene and might be a new therapeutic target of human glioblastoma.

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics*
  • Apoptosis
  • Base Sequence
  • Brain / metabolism
  • Brain / pathology
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / pathology
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic*
  • Glioblastoma / genetics*
  • Glioblastoma / pathology
  • Humans
  • MicroRNAs / genetics*

Substances

  • Adaptor Proteins, Signal Transducing
  • MIRN330 microRNA, human
  • MicroRNAs
  • SH3GL2 protein, human

Grants and funding

This work is supported by grants from Natural Science Foundation of China (Nos. 81171131, 81172197, 30973079, 81072056), the special fund for Scientific Research of Doctor-degree Subjects in Colleges and Universities, (Nos. 20092104110015, 20102104110009), Natural Science Foundation of Liaoning Province in China (No. 201102300), Science and Technology Plan Projects of Liaoning Province in China (No. 2011225020) and Shenyang Science and Technology Plan Projects (Nos. F-10-205-1-22, F-10-205-1-37). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.