10-Hydroxy-2-decenoic acid prevents ultraviolet A-induced damage and matrix metalloproteinases expression in human dermal fibroblasts

J Eur Acad Dermatol Venereol. 2013 Oct;27(10):1269-77. doi: 10.1111/j.1468-3083.2012.04707.x. Epub 2012 Oct 3.

Abstract

Background: 10-Hydroxy-2-decenoic acid (10-HDA) is a major fatty acid component of royal jelly, which has been reported to have a variety of beneficial pharmacological characteristics. However, the effects of 10-HDA on skin photoageing and its potential mechanism of action are unclear.

Objective: We investigated the protective effects of 10-HDA on ultraviolet (UV) A-induced damage in human dermal fibroblasts (HDFs). We then explored the inhibitory effects of 10-HDA on UVA-induced matrix metalloproteinases (MMPs) expression and elucidated the signalling pathways controlling MMPs inhibition.

Methods: Primary human dermal fibroblasts were exposed to UVA. Cell proliferation, cellular senescent state and collagen content were analysed using CCK-8, senescence-associated β-galactosidase staining and Sircol collagen assay, respectively. Fluorometric assays were performed to detect the formation of reactive oxygen species (ROS) in the cells. The mRNA levels of MMP-1, MMP-3 and type I (α1) collagen were determined by quantitative real-time PCR. Western blot was applied to detect the expression of MMP-1, MMP-3, JNK and p38 MAPK.

Results: HDFs treated with 10-HDA were significantly protected from UVA-induced cytotoxicity, ROS, cellular senescence and stimulated collagen production. Moreover, 10-HDA suppressed the UVA-induced expression of MMP-1 and MMP-3 at both the transcriptional and protein levels. Treatment with 10-HDA also reduced the UVA-induced activation of the JNK and p38 MAPK pathways.

Conclusion: The data obtained in this study provide evidence that 10-HDA could prevent UVA-induced damage and inhibit MMP-1 and MMP-3 expressions. Therefore, 10-HDA may be a potential agent for the prevention and treatment of skin photoageing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Proliferation / drug effects
  • Cell Proliferation / radiation effects
  • Cells, Cultured
  • Cellular Senescence / drug effects
  • Cellular Senescence / radiation effects
  • Child
  • Child, Preschool
  • Collagen / metabolism
  • Fatty Acids, Monounsaturated / pharmacology*
  • Fibroblasts / metabolism*
  • Fibroblasts / pathology
  • Fibroblasts / radiation effects*
  • Humans
  • MAP Kinase Signaling System / drug effects
  • MAP Kinase Signaling System / radiation effects
  • Male
  • Matrix Metalloproteinase 1 / metabolism*
  • Matrix Metalloproteinase 3 / metabolism*
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects
  • Signal Transduction / radiation effects
  • Sincalide / metabolism
  • Skin / metabolism*
  • Skin / pathology
  • Skin / radiation effects*
  • Ultraviolet Rays / adverse effects*
  • beta-Galactosidase / metabolism

Substances

  • Fatty Acids, Monounsaturated
  • Reactive Oxygen Species
  • 10-hydroxy-2-decenoic acid
  • Collagen
  • beta-Galactosidase
  • Matrix Metalloproteinase 3
  • Matrix Metalloproteinase 1
  • Sincalide