Beneficial effect of a sturgeon-based bioactive compound on gene expression of tumor necrosis factor-alpha, matrix metalloproteinases and type-10 collagen in human chondrocytes

J Biol Regul Homeost Agents. 2012 Jul-Sep;26(3):337-45.


In the present study, we examined the effect of a marine bioactive compound containing high-purity caviar-derived DNA, collagen elastin and protein extracts from sturgeon (LD-1227, Caviarlieri, Laboratoires Dom, Switzerland) on IL-1beta-induced activation and production of TNFalpha and MMP-13 in human osteo-arthritis (OA) chondrocytes and intracellular signaling factors. Human chondrocytes were derived from OA cartilage and stimulated with IL-1beta. Gene expression of TNFalpha, MMP-13, MMP-1 and Col10A1 was measured by quantitative RT-PCR. TNFalpha protein in culture medium was determined using cytokine-specific ELISA. Western immunoblotting was used to analyze the MMP-13 production in the culture medium and the activation of NF-kB. DNA binding activity of NF-kB p65 was determined using a highly sensitive and specific ELISA. MMP-13 activity in the culture medium was assayed by gelatine zymography. LD-1227 significantly decreased IL-1beta-stimulated gene expression and production of TNFalpha, MMP-1, MMP-13 and Col10A1 in human chondrocytes. The inhibitory effect of LD-1227 on the IL-1beta-induced expression of these genes was mediated at least in part via suppression of NF-kB p65. These data show that LD-1227 can inhibit IL-1beta-induced proliferation and inflammatory reactions via inhibited activation of the transcription factor NF-kB pathway in human chondrocytes derived from OA patients. These novel pharmacological actions of LD-1227 on IL-1beta-stimulated human OA chondrocytes provide suggestions that this marine biology compound may inhibit cartilage degradation by suppressing IL-1beta-mediated activation and the catabolic response in human chondrocytes.

Publication types

  • Clinical Trial

MeSH terms

  • Aged
  • Animals
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrocytes / metabolism*
  • Collagen Type X / biosynthesis*
  • Complex Mixtures / chemistry
  • Complex Mixtures / pharmacology*
  • Female
  • Fish Proteins / chemistry
  • Fish Proteins / pharmacology*
  • Fishes*
  • Gene Expression Regulation / drug effects*
  • Humans
  • Interleukin-1beta / biosynthesis
  • Male
  • Matrix Metalloproteinase 1 / biosynthesis*
  • Matrix Metalloproteinase 13 / biosynthesis*
  • Middle Aged
  • NF-kappa B / metabolism
  • Tumor Necrosis Factor-alpha / biosynthesis*


  • Collagen Type X
  • Complex Mixtures
  • Fish Proteins
  • IL1B protein, human
  • Interleukin-1beta
  • NF-kappa B
  • Tumor Necrosis Factor-alpha
  • MMP13 protein, human
  • Matrix Metalloproteinase 13
  • MMP1 protein, human
  • Matrix Metalloproteinase 1