Abstract
We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacteriocins / biosynthesis*
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Escherichia coli* / metabolism
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Molecular Sequence Data
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Peptides
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Bacteriocins
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Peptides
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gardimycin