Digital confocal microscope

Opt Express. 2012 Sep 24;20(20):22720-7. doi: 10.1364/OE.20.022720.


We demonstrate experimentally a scanning confocal microscopy technique based on digital holography. The method relies on digital holographic recording of the scanned spot. The data collected in this way contains all the necessary information to digitally produce three-dimensional images. Several methods to treat the data are presented. Examples of reflection and transmission images of epithelial cells and mouse brain tissue are shown.

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Holography / instrumentation*
  • Image Enhancement / instrumentation*
  • Image Interpretation, Computer-Assisted / instrumentation*
  • Imaging, Three-Dimensional / instrumentation*
  • Microscopy, Confocal / instrumentation*
  • Signal Processing, Computer-Assisted / instrumentation*