Further replication studies of the EVE Consortium meta-analysis identifies 2 asthma risk loci in European Americans

J Allergy Clin Immunol. 2012 Dec;130(6):1294-301. doi: 10.1016/j.jaci.2012.07.054. Epub 2012 Oct 4.


Background: Genome-wide association studies of asthma have implicated many genetic risk factors, with well-replicated associations at approximately 10 loci that account for only a small proportion of the genetic risk.

Objectives: We aimed to identify additional asthma risk loci by performing an extensive replication study of the results from the EVE Consortium meta-analysis.

Methods: We selected 3186 single nucleotide polymorphisms for replication based on the P values from the EVE Consortium meta-analysis. These single nucleotide polymorphisms were genotyped in ethnically diverse replication samples from 9 different studies, totaling 7202 cases, 6426 controls, and 507 case-parent trios. Association analyses were conducted within each participating study, and the resulting test statistics were combined in a meta-analysis.

Results: Two novel associations were replicated in European Americans: rs1061477 in the KLK3 gene on chromosome 19 (combined odds ratio = 1.18; 95% CI, 1.10-1.25) and rs9570077 (combined odds ratio =1.20; 95% CI, 1.12-1.29) on chromosome 13q21. We could not replicate any additional associations in the African Americans or Latinos.

Conclusions: This extended replication study identified 2 additional asthma risk loci in populations of European descent. The absence of additional loci for African Americans and Latinos highlights the difficulty in replicating associations in admixed populations.

Publication types

  • Multicenter Study
  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Asthma / epidemiology*
  • Asthma / genetics*
  • Asthma / immunology
  • Black or African American
  • Chromosomes, Human, Pair 19 / genetics*
  • DNA Mutational Analysis
  • Genetic Loci / immunology
  • Genetic Predisposition to Disease
  • Genome-Wide Association Study
  • Hispanic or Latino
  • Humans
  • Kallikreins / genetics*
  • Meta-Analysis as Topic
  • Polymorphism, Single Nucleotide
  • Prostate-Specific Antigen / genetics*
  • Risk Factors
  • United States
  • White People


  • KLK3 protein, human
  • Kallikreins
  • Prostate-Specific Antigen

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